The bitterness of neem is due to the occurrence of limonoids, which are the
tetranor¬triterpenoids based (on apo-tirucallol) skeleton87. The term limonoids is derived from
limonin, the first -tetranortriterpenoids obtained from citrus bitter principles in 1841, the structure
of which could be established only 1960151. This class of compounds confined to the order
Rutales comprising Meliaceae, Cneoraceae, Rutaceae and Simaroubaceae, the last family
catabolising another class called quassinoids. The limonoids occurring in Meliaceae are also
known as meliacins. Out of over 300 limonoids known to-day, about one-third is accounted by
neem (Azadirachta indica) and Chinaberry (Melia azedarach) alone.
Pure Neem Oil bitter principles can be conveniently classified under eight groups:
protomeliacins. limonoids with a modified side chain (e.g. y-hydroxybutenolides), azadirone and
its derivatives. gedunin and its derivatives. vi/asinin type compounds, and those belonging to
three C-seco¬meliacins-nimbin. salannin and azadirachtin.